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JoVE Journal Genetics
Under anvendelse af et fluorescerende PCR-kapillær gelelektroforese Teknik til genotype CRISPR / Cas9-medieret Knockout Mutanter i et format med høj kapacitet
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Under anvendelse af et fluorescerende PCR-kapillær gelelektroforese Teknik til genotype CRISPR / Cas9-medieret Knockout Mutanter i et format med høj kapacitet

Article DOI: 10.3791/55586-v • 08:25 min • April 8th, 2017
April 8th, 2017 •
1, 1, 1, 1
1Cancer & Stem Cell Biology Programme, Duke-NUS Medical School

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Genotypebestemmelse teknikken beskrevet her, som kobler fluorescerende polymerasekædereaktion (PCR) til kapillær gelelektroforese muliggør high-throughput genotyping af nuklease-medieret knockout kloner. Den omgår begrænsninger af andre genotypebestemmelsesteknikker står og er mere omkostningseffektive end sekventeringsfremgangsmåder.

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Genetik Genom redigering knockout indsættelse / deletionsmutation high-throughput screening direkte lysis multiplexable
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