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Journal / Developmental Biology / Elektrofysiologisk analyse af humane pluripotente …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Elektrofysiologisk analyse af humane pluripotente stamceller-afledte kardiomyocytter (hPSC-CM'er) ved anvendelse af multi-elektrode arrays (MEA'er)
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Elektrofysiologisk analyse af humane pluripotente stamceller-afledte kardiomyocytter (hPSC-CM'er) ved anvendelse af multi-elektrode arrays (MEA'er)

Article DOI: 10.3791/55587-v • 11:13 min • May 12th, 2017
May 12th, 2017 •
1, 1, 1, 1,2, 1
1Department of Anatomy and Embryology, Leiden University Medical Center, 2Department of Applied Stem Cell Technologies, University of Twente

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Elektrofysiologisk karakterisering af kardiomyocytter afledt af humane pluripotente stamceller (hPSC-CM'er) er afgørende for hjertesygdomsmodellering og til bestemmelse af lægemiddelresponser. Denne protokol tilvejebringer de nødvendige oplysninger til at dissociere og plader hPSC-CM'er på multi-elektrode arrays, måle deres feltpotentiale og en metode til analyse af QT- og RR-intervaller.

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Udviklingsbiologi udgave 123 humane pluripotente stamcellerafledte cardiomyocytter (hPSC-CM'er) mikroelektrodearrayer multielektrodearrayer feltpotentiale QT-interval RR-interval lægemiddel screening hjertearytmi langt QT-syndrom sikkerhedsfarmakologi Elektrofysiologi
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