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Immunology and Infection

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JoVE Journal Immunology and Infection
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
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A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Article DOI: 10.3791/55591-v • 07:35 min • July 5th, 2017
July 5th, 2017 •
1, 2, 2, 2,3,4,5, 1
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute, 3Singapore National Eye Center, 4Duke-NUS Medical School, 5Yong Loo Lin School of Medicine

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Summary
Automatic Translation

The exposed normal ocular surface consists of cornea and conjunctiva. Epithelial cells, goblet cells and immune cells are present in the conjunctiva. Here, a non-invasive, technique of impression cytology is described using an impression cytology device and flow cytometry to analyze immune cells in the conjunctiva.

Tags

Impression Cytology Technique Isolate Cells Phenotype Cells Conjunctiva Ocular Surface Disease Non-invasive Clinic And Lab Immune Cells Ocular Surface Inflammation Therapy Of Ocular Surface Inflammatory Disorders Specific Populations Of Immune Cells Ocular Surface Immune Cell Population Ocular Toxicology Ocular Fibrosis Low Number Of Cells Collected From The Eye Topical Anesthesia Superior Bulbar Conjunctiva Inferior Fornix Impression Cytology Device Harvest Ocular Membrane
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