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Journal / Immunology and Infection / En ikke-invasiv måde at isolere og fænotype…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
En ikke-invasiv måde at isolere og fænotype celler fra konjunktiva
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En ikke-invasiv måde at isolere og fænotype celler fra konjunktiva

Article DOI: 10.3791/55591-v • 07:35 min • July 5th, 2017
July 5th, 2017 •
1, 2, 2, 2,3,4,5, 1
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute, 3Singapore National Eye Center, 4Duke-NUS Medical School, 5Yong Loo Lin School of Medicine

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Den udsatte normale okulære overflade består af hornhinde og bindehinden. Epitelceller, boblerceller og immunceller er til stede i bindehinden. Her beskrives en ikke-invasiv teknik med indtrykcytologi ved anvendelse af en indtrykcytologienhed og flowcytometri til analyse af immunceller i bindehinden.

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Immunologi udgave 125 Impression cytologi tør øjne sygdom indtryk cytologi enhed effektor hukommelse T-celler central hukommelse T-celler terminalt differentieret effektor hukommelse T-celler HLA-DR okulær overflade impression cytologi flow cytometry
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