Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Spændingsklemme Fluorometri i
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Spændingsklemme Fluorometri i
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Journal (Biochemistry)
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
Journal (Biochemistry)
Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
 
Click here for the English version

Spændingsklemme Fluorometri i

Article DOI: 10.3791/55598-v • 09:24 min • May 27th, 2017
May 27th, 2017 •
1, 1,2
1Department of Pharmacology and Physiology, University of Montréal, 2Department of Physics, University of Montréal

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne artikel beskriver en forbedring af konventionel spændingsklemmefluorometri (VCF), hvor fluorescerende unaturlige aminosyrer (fUAA) anvendes i stedet for maleimidfarvestoffer til sondring af strukturelle omlejringer i ionkanaler. Fremgangsmåden indbefatter Xenopus oocyt DNA-injektion, RNA / fUAA-coinjection og samtidige strøm- og fluorescensmålinger.

Tags

Biochemistry Fluorescerende unaturlige aminosyrer spændingsklemmefluorometri åben oocytspændingsklemme Anap Amber Stop-codon-suppression DNA-mikroinjektion,
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter