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Høj opløsning Imaging og analyse af de enkelte Astral mikrotubulusdynamik i Spirende Gær
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Høj opløsning Imaging og analyse af de enkelte Astral mikrotubulusdynamik i Spirende Gær

Article DOI: 10.3791/55610-v • 10:23 min • April 20th, 2017
April 20th, 2017 •
1, 1, 1
1Department of Cell and Developmental Biology, University of Colorado School of Medicine

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Knopskydende gær er et fordelagtigt model til undersøgelse mikrotubulusdynamik in vivo på grund af sin stærke genetik og dens enkle mikrotubuluscytoskelettet. Den følgende protokol beskriver, hvordan man omdanne og kultur gærceller, erhverve konfokal mikroskopi billeder, og kvantitativt analysere mikrotubulusdynamik i levende gærceller.

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Cellular Biology Cytoskeleton mikrotubulusdynamik Spirende gær mikroskopi live-cell imaging
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