Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / FRET de alta precisión a nivel de molécula única…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
FRET de alta precisión a nivel de molécula única para la determinación de estructuras de biomoléculas
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Journal (Biochemistry)
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE
Journal (Biochemistry)
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE
 
Click here for the English version

FRET de alta precisión a nivel de molécula única para la determinación de estructuras de biomoléculas

Article DOI: 10.3791/55623-v • 11:24 min • May 13th, 2017
May 13th, 2017 •
1, 2, 2, 3, 3, 2
1Department of Chemistry, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences, University of Texas Health Science Center

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Aquí se presenta un protocolo para experimentos FRET de alta precisión en el nivel de una sola molécula. Además, esta metodología puede usarse para identificar tres estados conformacionales en el dominio de unión al ligando del receptor de N-metil-D-aspartato (NMDA). Determinar las distancias exactas es el primer paso hacia la construcción de modelos estructurales basados ​​en experimentos FRET.

Tags

Bioquímica Edición 123 FRET molécula única distancia interdye ADN fluoróforo detección de fluorescencia multiparamétrica excitación intercalada pulsada etiquetado de proteínas
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter