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Biochemistry

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Måling af basal og forskolin-stimuleret lipolyse i fedtpads

Article DOI: 10.3791/55625-v • 07:59 min • July 21st, 2017
July 21st, 2017 •
1, 1
1School of Pharmacy, University of Wyoming

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Denne protokol beskriver metoden til bestemmelse af basal og forskolinstimuleret lipolyse i fedtpuder, der opnås fra normal chow diet (NCD) eller fedtsyre (HFD) ± capsaicin fodret vildtype-mus. Som et indeks for lipolyse blev glycerolfrigivelse målt fra inguinal adipose fatpads.

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Biokemi udgave 125 lipolyse hvidt fedtvæv fedtpuder af fedtindhold glycerolfrigivelse forskolin capsaicin TRPV1.
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