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Journal / Cancer Research / Harnessing DNA Dye-utløst Side Population…
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Cancer Research

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JoVE Journal Cancer Research
Harnessing DNA Dye-utløst Side Population Fenotype for å oppdage og rense kreft stamceller fra biologiske prøver
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Harnessing DNA Dye-utløst Side Population Fenotype for å oppdage og rense kreft stamceller fra biologiske prøver

Article DOI: 10.3791/55634-v • 09:57 min • May 10th, 2017
May 10th, 2017 •
*1,2,3, 2,3, 2,3,4, 2, *2,3
1Institute of Immunobiology, Kantonsspital St. Gallen, 2Internal Medicine V, Medical University of Innsbruck, 3Tyrolean Cancer Research Institute (TKFI), 4Medical Clinic III, Oncology, Hematology, Immunoncology and Rheumatology, University Clinic Bonn (UKB)
* These authors contributed equally

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Metoder som tillater karakterisering og isolering av stamcellepopulasjoner fra biologiske prøver, er kritiske for fremveksten av stamcelle-målrettede behandlinger i kreft og utover. Her gir vi en detaljert protokoll for kreft-stamcelleisolasjon ved hjelp av fargestoff-utløst sidepopulasjonsfenotype.

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Cancer Research Utgave 123 Sidepopulasjon Flowcytometri FACS Kreftstamcelle Kreft Stamcelle Legemiddeltransportør ABCG2 ABCB1
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