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Biology
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将细胞质Foci分类为哺乳动物应激颗粒的方法
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Article
DOI:
10.3791/55656-v
•
09:33 min
•
May 12th, 2017
将细胞质Foci分类为哺乳动物应激颗粒的方法
May 12th, 2017
•
Anaïs Aulas*1,2, Marta M. Fay*1,2, Witold Szaflarski1,2,3, Nancy Kedersha1,2, Paul Anderson1,2, Pavel Ivanov1,2,4
1Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, 2Department of Medicine, Harvard Medical School, 3Department of Histology and Embryology, Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
应激颗粒(SG)是在暴露于各种应激的细胞中形成的非膜细胞质结构。 SGs包含mRNA,RNA结合蛋白,小核糖体亚基,翻译相关因子和各种细胞信号传导蛋白。该协议描述了一种使用几种实验方法来检测,表征和量化真正的 SG的工作流程。