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JoVE Journal
Biology

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将细胞质Foci分类为哺乳动物应激颗粒的方法
 
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将细胞质Foci分类为哺乳动物应激颗粒的方法

Article DOI: 10.3791/55656-v 09:33 min May 12th, 2017
May 12th, 2017

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

应激颗粒(SG)是在暴露于各种应激的细胞中形成的非膜细胞质结构。 SGs包含mRNA,RNA结合蛋白,小核糖体亚基,翻译相关因子和各种细胞信号传导蛋白。该协议描述了一种使用几种实验方法来检测,表征和量化真正的 SG的工作流程。

Tags

细胞生物学,第123期,RNA颗粒,应激颗粒,mRNA,翻译抑制,免疫染色,
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