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JoVE Journal Biology
Methoden om Cytoplasmatische Foci te classificeren als Mammalian Stress Granules
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Methoden om Cytoplasmatische Foci te classificeren als Mammalian Stress Granules

Article DOI: 10.3791/55656-v • 09:33 min • May 12th, 2017
May 12th, 2017 •
*1,2, *1,2, 1,2,3, 1,2, 1,2, 1,2,4
1Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, 2Department of Medicine, Harvard Medical School, 3Department of Histology and Embryology, Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.
* These authors contributed equally

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Stress Granules (SG's) zijn niet-membraan cytoplasmatische structuren die in cellen vormen die blootstaan ​​aan een verscheidenheid aan stressen. SG's bevatten mRNA's, RNA-bindende eiwitten, kleine ribosomale subeenheden, vertaalgerelateerde factoren en diverse cel signalerende eiwitten. Dit protocol beschrijft een workflow die gebruik maakt van verschillende experimentele benaderingen om bona fide SG's te detecteren, karakteriseren en kwantificeren.

Tags

Cellular Biology RNA granules stress granules mRNA translation repression immunostaining, stressrespons
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