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Journal / Biology / Metoder för att klassificera cytoplasmiska foci…
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Metoder för att klassificera cytoplasmiska foci som däggdjursstresskorn
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Metoder för att klassificera cytoplasmiska foci som däggdjursstresskorn

Article DOI: 10.3791/55656-v • 09:33 min • May 12th, 2017
May 12th, 2017 •
*1,2, *1,2, 1,2,3, 1,2, 1,2, 1,2,4
1Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, 2Department of Medicine, Harvard Medical School, 3Department of Histology and Embryology, Poznan University of Medical Sciences, 4The Broad Institute of Harvard and M.I.T.
* These authors contributed equally

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Stress Granuler (SGs) är icke-membranformiga cytoplasmatiska strukturer som bildas i celler utsatta för en mängd stress. SG innehåller mRNA, RNA-bindande proteiner, små ribosomala subenheter, translationsrelaterade faktorer och olika cellsignalproteiner. I det här protokollet beskrivs ett arbetsflöde som använder flera experimentella metoder för att upptäcka, karakterisera och kvantifiera bona fide SG.

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Cellbiologi utgåva 123 RNA-granuler stressgranuler mRNA translationsundertryckning immunfärgning, stressrespons
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