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Flash-e-Freeze
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Flash-e-Freeze: una tecnica innovativa per l'acquisizione di membrana Dynamics con microscopia elettronica

Article DOI: 10.3791/55664-v • 10:01 min • May 1st, 2017
May 1st, 2017 •
*1,2, *1, 1,3
1Department of Cell Biology, Johns Hopkins School of Medicine, 2Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 3Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
* These authors contributed equally

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Abbiamo sviluppato una nuova tecnica di microscopia elettronica, "flash-e-congelamento" che permette la visualizzazione di dinamica della membrana con ms risoluzione temporale. Questa tecnica combina la stimolazione optogenetic di neuroni con alta pressione di congelamento. Qui, dimostriamo le procedure e descrivere i protocolli in dettaglio.

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Biologia Cellulare Numero 123 microscopia elettronica optogenetics sinapsi la dinamica della membrana congelare la sostituzione channelrhodopsin ad alta pressione di congelamento esocitosi endocitosi microscopia elettronica risolta nel tempo flash-e-freeze
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