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Journal / Biology / Flash-e-Freeze: uma nova técnica para capturar…
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Biology

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JoVE Journal Biology
Flash-e-Freeze
Transverse Aortic Constriction in Mice
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Transverse Aortic Constriction in Mice
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Journal (Biology)
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Flash-e-Freeze: uma nova técnica para capturar Membrane Dynamics com Electron Microscopy

Article DOI: 10.3791/55664-v • 10:01 min • May 1st, 2017
May 1st, 2017 •
*1,2, *1, 1,3
1Department of Cell Biology, Johns Hopkins School of Medicine, 2Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 3Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
* These authors contributed equally

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Nós desenvolvemos uma nova técnica de microscopia electrónica, de "flash-e-congelamento", que permite a visualização da dinâmica da membrana com ms resolução temporal. Esta técnica combina a estimulação optogenetic de neurónios com congelamento de alta pressão. Aqui, demonstramos os procedimentos e descrever os protocolos em detalhe.

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Cellular Biology Edição 123 microscopia electrónica Optogenetics sinapse a dinâmica da membrana congelar substituição channelrodopsina congelamento de alta pressão exocitose endocitose microscopia electrónica resolvida no tempo de flash-e-congelamento
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