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Journal / Neuroscience / Analyse til måling af nukleocytoplasmisk…
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JoVE Journal Neuroscience
Analyse til måling af nukleocytoplasmisk transport i realtid inden for motor neuronlignende NSC-34 celler
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Analyse til måling af nukleocytoplasmisk transport i realtid inden for motor neuronlignende NSC-34 celler

Article DOI: 10.3791/55676-v • 08:53 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1,2
1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev

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Denne protokol beskriver en metode til sensitivt at måle den nukleocytoplasmatiske transportrate inden for motorneuronlignende NSC-34-celler ved at kvantificere den realtidsændring i nuklearimporten af ​​et NLS-NES-GFP-protein.

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Neurovidenskab udgave 123 nukleocytoplasmisk transport shuttle-GFP NSC-34-celler C9orf72 ALS
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