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Ensaio para Medir o Transporte Nucleocitoplasmático em Tempo Real dentro de Células NSC-34 do tipo Motor-Neuron
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Ensaio para Medir o Transporte Nucleocitoplasmático em Tempo Real dentro de Células NSC-34 do tipo Motor-Neuron

Article DOI: 10.3791/55676-v • 08:53 min • May 16th, 2017
May 16th, 2017 •
1, 1, 1,2
1Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev

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Este protocolo descreve um método para medir sensivelmente a taxa de transporte nucleocitoplasmático dentro de células NSC-34 do tipo neurônio motor, quantificando a mudança em tempo real na importação nuclear de uma proteína NLS-NES-GFP.

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Neurociência Edição 123 transporte nucleocitoplasmático GFP de transporte células NSC-34 C9orf72 ALS
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