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Journal / Biology / Mixed colture primarie di murino tenue…
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Mixed colture primarie di murino tenue finalizzate allo studio di Gut secrezione dell'ormone e imaging cellulare dal vivo di celle enteroendocrine
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Mixed colture primarie di murino tenue finalizzate allo studio di Gut secrezione dell'ormone e imaging cellulare dal vivo di celle enteroendocrine

Article DOI: 10.3791/55687-v • 09:16 min • April 20th, 2017
April 20th, 2017 •
*1, *1, 1, 1, 1
1Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge
* These authors contributed equally

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Questo protocollo descrive l'isolamento e la coltura di piccole cellule intestinali murine misti. Queste colture primarie intestinali consentono la ricerca di vie di segnalazione sottostanti intestinale secrezione peptide utilizzando un numero di tecniche.

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Biologia cellulare intestino tenue il duodeno topo primaria cultura enteroendocrine cellule L intestino ormone
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