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Gemischte Primärkulturen von Murine Duenndarm bestimmt zur Untersuchung von Gut Hormonausschüttung und Live Cell Imaging von enteroendokrinen Zellen
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Gemischte Primärkulturen von Murine Duenndarm bestimmt zur Untersuchung von Gut Hormonausschüttung und Live Cell Imaging von enteroendokrinen Zellen

Article DOI: 10.3791/55687-v • 09:16 min • April 20th, 2017
April 20th, 2017 •
*1, *1, 1, 1, 1
1Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge
* These authors contributed equally

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Dieses Protokoll beschreibt die Isolierung und Kultur von gemischten murine kleinen Darmzellen. Diese primären intestinalen Kulturen ermöglichen die Untersuchung von Signalwegen zugrunde liegenden Darmpeptid die Sekretion einer Reihe von Techniken.

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Cellular Biology Ausgabe 122 Dünndarm Zwölffingerdarm Maus primärer Kultur enteroendokrinen L-Zelle Darm Hormon
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