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Journal / Developmental Biology / Hele-celle Patch-clamp Opptak av isolerte primære …
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Developmental Biology

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JoVE Journal Developmental Biology
Hele-celle Patch-clamp Opptak av isolerte primære epitelceller fra epididymene
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Hele-celle Patch-clamp Opptak av isolerte primære epitelceller fra epididymene

Article DOI: 10.3791/55700-v • 11:52 min • August 3rd, 2017
August 3rd, 2017 •
1,2,3, 1,2,3, 1,3, 4, 1
1School of Life Science and Technology, ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

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Vi presenterer en protokoll som kombinerer celleisolasjon og helcelle patch-clamp opptak for å måle de elektriske egenskapene til de primære dissocierte epitelceller fra rotte cauda-epididymider. Denne protokollen tillater undersøkelse av funksjonelle egenskaper til primære epididymepitelceller for å belyse epididymis fysiologiske rolle.

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Utviklingsbiologi utgave 126 Helecellepatchklemme enkeltcelleopptak nåværende klemme spenningsklemme elektrofysiologi av celler passive membranegenskaper hvilemembranpotensiale primære epitelceller hovedceller epididymis.
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