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JoVE Journal
Developmental Biology

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Hele-celle patch-clamp optagelser af isolerede primære epitelceller fra epididymis
 
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Hele-celle patch-clamp optagelser af isolerede primære epitelceller fra epididymis

Article DOI: 10.3791/55700
August 3rd, 2017

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Summary August 3rd, 2017

Please note that all translations are automatically generated.

Click here for the English version.

Vi præsenterer en protokol, der kombinerer cellisolering og fuldcelle patch-clamp-optagelse til måling af de primære dissocierede epitelcellers elektriske egenskaber fra rotte-cauda-epididymiderne. Denne protokol tillader undersøgelse af de primære epididymepitelcellers funktionelle egenskaber for yderligere at belyse epididymis fysiologiske rolle.

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