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Journal / Developmental Biology / Hele-celle patch-clamp optagelser af isolerede…
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Developmental Biology

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JoVE Journal Developmental Biology
Hele-celle patch-clamp optagelser af isolerede primære epitelceller fra epididymis
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Hele-celle patch-clamp optagelser af isolerede primære epitelceller fra epididymis

Article DOI: 10.3791/55700-v • 11:52 min • August 3rd, 2017
August 3rd, 2017 •
1,2,3, 1,2,3, 1,3, 4, 1
1School of Life Science and Technology, ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

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Vi præsenterer en protokol, der kombinerer cellisolering og fuldcelle patch-clamp-optagelse til måling af de primære dissocierede epitelcellers elektriske egenskaber fra rotte-cauda-epididymiderne. Denne protokol tillader undersøgelse af de primære epididymepitelcellers funktionelle egenskaber for yderligere at belyse epididymis fysiologiske rolle.

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Udviklingsbiologi Udgave 126 Helecelle patch-klemme enkeltcelleoptagelse strømklemme spændingsklemme elektrofysiologi af celler passive membranegenskaber hvilemembranpotentiale primære epithelceller hovedceller epididymier.
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