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JoVE Journal Biochemistry
Detectie en visualisatie van DNA-schade-geïnduceerde eiwitcomplexen in suspensiecellenculturen met behulp van de proximity-liggingsassay
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Journal (Biochemistry)
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Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
Journal (Biochemistry)
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
 
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Detectie en visualisatie van DNA-schade-geïnduceerde eiwitcomplexen in suspensiecellenculturen met behulp van de proximity-liggingsassay

Article DOI: 10.3791/55703-v • 13:10 min • June 9th, 2017
June 9th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Pathology, Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

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Hier wordt aangetoond hoe de in situ Proximity Ligation Assay (PLA) kan worden gebruikt om de directe eiwit-eiwit interacties tussen ATM en p53 te detecteren en visualiseren in suspensiecellen die blootgesteld zijn aan genotoxische stress.

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Biochemie Uitgave 124 DNA-schade DNA-reparatie ATM p53 suspensiecelcultuur BCR-ABL + B-cel acute lymfoblastische leukemie eiwit-eiwit interactie,
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