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Journal / Biochemistry / Oligopeptide Concurrentie Assay voor Fosforylatie …
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Biochemistry

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JoVE Journal Biochemistry
Oligopeptide Concurrentie Assay voor Fosforylatie Site Bepaling
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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
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Oligopeptide Concurrentie Assay voor Fosforylatie Site Bepaling

Article DOI: 10.3791/55708-v • 09:16 min • May 18th, 2017
May 18th, 2017 •
1, 1, 2, 2, 1
1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

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Peptide competitiebepalingen worden veel gebruikt in een verscheidenheid aan moleculaire en immunologische experimenten. Dit document beschrijft een gedetailleerde methode voor een in vitro oligopeptide-concurrerende kinase-analyse en de bijbehorende validatieprocedures, die nuttig kunnen zijn om specifieke fosforyleringsplaatsen te vinden.

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Biochemie uitgave 123, peptide-inhibitor fosforylering AMPK Nrf2 consensusmotief plaatsgerichte mutagenese
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