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Biochemistry

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JoVE Journal Biochemistry
Oligopeptid-Konkurrenz-Assay zur Phosphorylierungsbestimmung
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Oligopeptid-Konkurrenz-Assay zur Phosphorylierungsbestimmung

Article DOI: 10.3791/55708-v • 09:16 min • May 18th, 2017
May 18th, 2017 •
1, 1, 2, 2, 1
1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

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Peptid-Konkurrenz-Assays sind weit verbreitet in einer Vielzahl von molekularen und immunologischen Experimenten verwendet. Dieses Papier beschreibt eine detaillierte Methode für einen in vitro- Oligopeptid-konkurrierenden Kinase-Assay und die damit verbundenen Validierungsverfahren, die nützlich sein können, um spezifische Phosphorylierungsstellen zu finden.

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Biochemie Ausgabe 123, Peptidinhibitor Phosphorylierung AMPK Nrf2 Konsensusmotiv ortsgerichtete Mutagenese
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