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Journal / Biochemistry / Oligopeptidkonkurranseanalyse for…
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Biochemistry

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JoVE Journal Biochemistry
Oligopeptidkonkurranseanalyse for fosforyleringsstedbestemmelse
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Oligopeptidkonkurranseanalyse for fosforyleringsstedbestemmelse

Article DOI: 10.3791/55708-v • 09:16 min • May 18th, 2017
May 18th, 2017 •
1, 1, 2, 2, 1
1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

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Peptidkonkurranseanalyser er mye brukt i en rekke molekylære og immunologiske eksperimenter. Dette papiret beskriver en detaljert metode for en in vitro oligopeptid-konkurrerende kinaseanalyse og de tilhørende valideringsprosedyrer, som kan være nyttige for å finne spesifikke fosforyleringssteder.

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Biokjemi utgave 123, peptidinhibitor fosforylering AMPK Nrf2 konsensusmotiv stedrettet mutagenese
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