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Journal / Biochemistry / Oligopeptidkompetitionsanalys för…
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Biochemistry

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JoVE Journal Biochemistry
Oligopeptidkompetitionsanalys för fosforyleringsställningsbestämning
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Journal (Biochemistry)
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
Journal (Biochemistry)
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
 
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Oligopeptidkompetitionsanalys för fosforyleringsställningsbestämning

Article DOI: 10.3791/55708-v • 09:16 min • May 18th, 2017
May 18th, 2017 •
1, 1, 2, 2, 1
1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University

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Peptidkonkurrensanalyser används ofta i ett flertal molekylära och immunologiska experiment. Detta papper beskriver en detaljerad metod för en in vitro oligopeptidkompetent kinasassay och de associerade valideringsförfarandena, som kan vara användbara för att hitta specifika fosforyleringsställen.

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Biochemistry , peptidinhibitor fosforylering AMPK Nrf2 konsensusmotif ställdriktad mutagenes
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