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Journal / Cancer Research / Ex Vivo Avbildning av bosatt CD8 T-lymfocytter i…
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Cancer Research

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JoVE Journal Cancer Research
Ex Vivo Avbildning av bosatt CD8 T-lymfocytter i menneskelig lunge svulst skiver bruker AC Confocal mikroskopi
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Ex Vivo Avbildning av bosatt CD8 T-lymfocytter i menneskelig lunge svulst skiver bruker AC Confocal mikroskopi

Article DOI: 10.3791/55709-v • 08:28 min • December 27th, 2017
December 27th, 2017 •
*1, *1, *1, 2,3, 4, 2,3, 1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally

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Denne protokollen beskriver en metode for å bilde bosatt svulst-infiltrere CD8 T celler merket med fluorescently kombinert antistoffer i menneskelig lunge svulst skiver. Denne teknikken tillater sanntid analyser av CD8 T celle overføring ved hjelp av AC confocal mikroskopi.

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Kreftforskning problemet 130 Tumor immunologi menneske lunge svulst organotypic skiver T celle migrasjon fluorescens mikroskopi AC confocal
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