JoVE Journal
Cancer Research
Cancer Research
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Ex Vivoひと肺腫瘍内常駐 cd8 陽性 T リンパ球の画像スライス共焦点顕微鏡を用いた
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Article
DOI:
10.3791/55709-v
•
08:28 min
•
December 27th, 2017
Ex Vivoひと肺腫瘍内常駐 cd8 陽性 T リンパ球の画像スライス共焦点顕微鏡を用いた
December 27th, 2017
•
Elisa Peranzoni*1, Houcine Bougherara*1, Sarah Barrin*1, Audrey Mansuet-Lupo2,3, Marco Alifano4, Diane Damotte2,3, Emmanuel Donnadieu1
1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
このプロトコルでは、イメージの居住者腫瘍浸潤 CD8 T 細胞ひと肺腫瘍スライス内結合蛍光抗体で標識する方法について説明します。この手法は、共焦点顕微鏡を用いた CD8 T 細胞の移動の実時間解析を許可します。