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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering af Side Population i Myc-induceret T-celle akut lymfoblastisk leukæmi i zebrafisk
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Isolering af Side Population i Myc-induceret T-celle akut lymfoblastisk leukæmi i zebrafisk

Article DOI: 10.3791/55711-v • 09:06 min • May 4th, 2017
May 4th, 2017 •
1,2, 1, 1, 1
1Department of Pediatrics, Section of Hematology-Oncology, The University of Chicago, 2Department of Biology and Biotechnology, Worcester Polytechnic Institute

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Her beskriver vi en teknik til isolering af sidebefolkningscellerne fra en zebrafiskmodel af myc-induceret T-celle akut lymfoblastisk leukæmi (T-ALL). Denne sidebestemmelsesanalyse er meget følsom og er beskrevet for zebrafisk T-ALL, men den kan være anvendelig for andre maligne og ikke-maligne zebrafiskcelletyper.

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Immunologi side befolkning flowcytometri T-celle akut lymfoblastisk leukæmi zebrafisk tol2-transgenese leukæmi initierende celle
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