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JoVE Journal Developmental Biology
Differentierende chondrocytter fra perifert blodafledte humane inducerede pluripotente stamceller
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Differentierende chondrocytter fra perifert blodafledte humane inducerede pluripotente stamceller

Article DOI: 10.3791/55722-v • 07:51 min • July 18th, 2017
July 18th, 2017 •
2, 1, 3, 1
1Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University

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Vi præsenterer en protokol for at generere en chondrogen afstamning fra humant perifert blod (PB) via inducerede pluripotente stamceller (iPSCs) ved hjælp af en integrationsfri metode, der omfatter embryoidlegem (EB) dannelse, ekspansion af fibroblast celler og chondrogen induktion.

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Udviklingsbiologi udgave 125 inducerede pluripotente stamceller perifert blod differentiering embryoidkrop fibroblastiske celler chondrocyt
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