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Journal / Developmental Biology / Unterscheidung von Chondrozyten aus periphere…
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Developmental Biology

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JoVE Journal Developmental Biology
Unterscheidung von Chondrozyten aus periphere Blut-abgeleiteten menschlichen induzierten pluripotenten Stammzellen
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
Journal (Developmental Biology)
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
Journal (Developmental Biology)
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
 
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Unterscheidung von Chondrozyten aus periphere Blut-abgeleiteten menschlichen induzierten pluripotenten Stammzellen

Article DOI: 10.3791/55722-v • 07:51 min • July 18th, 2017
July 18th, 2017 •
2, 1, 3, 1
1Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University

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Wir präsentieren ein Protokoll zur Erzeugung einer chondrogenen Linie aus menschlichem peripherem Blut (PB) über induzierte pluripotenten Stammzellen (iPSCs) unter Verwendung einer integrationsfreien Methode, die Embryoidkörperbildung (EB), Fibroblastische Zellenexpansion und chondrogene Induktion umfasst.

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Entwicklungsbiologie Ausgabe 125 induzierte pluripotenten Stammzellen peripheres Blut Differenzierung Embryoidkörper fibroblastische Zellen Chondrozyten
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