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JoVE Journal Developmental Biology
Differenziare i condrociti da cellule staminali pluripotenti indotte dall'uomo derivato dal sangue
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Differenziare i condrociti da cellule staminali pluripotenti indotte dall'uomo derivato dal sangue

Article DOI: 10.3791/55722-v • 07:51 min • July 18th, 2017
July 18th, 2017 •
2, 1, 3, 1
1Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University

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Presentiamo un protocollo per generare una linea condrogenica dal sangue periferico umano (PB) attraverso cellule staminali pluripotenti indotte (iPSCs) utilizzando un metodo senza integrazione, che comprende la formazione del corpo embrionale (EB), l'espansione delle cellule fibroblasiche e l'induzione condrogenica.

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Biologia dello sviluppo Numero 125 indotta da cellule staminali pluripotenti sangue periferico differenziazione corpo embrionale cellule fibroblastiche condrociti
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