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Condrócitos diferenciadores de células-tronco pluripotentes induzidas por humanos derivadas de sangue periférico
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Condrócitos diferenciadores de células-tronco pluripotentes induzidas por humanos derivadas de sangue periférico

Article DOI: 10.3791/55722-v • 07:51 min • July 18th, 2017
July 18th, 2017 •
2, 1, 3, 1
1Department of Orthopedics, Beijing Chao-Yang Hospital, Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology, Tongji University

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Apresentamos um protocolo para gerar uma linhagem condrogênica de sangue periférico humano (PB) através de células-tronco pluripotentes induzidas (iPSCs) usando um método livre de integração, que inclui a formação de corpo embrionóide (EB), expansão de células fibroblásticas e indução condrogênica.

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Biologia do Desenvolvimento Número 125 células-tronco pluripotentes induzidas sangue periférico diferenciação corpo embrionóide células fibroblásticas condrócitos
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