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Journal / Developmental Biology / 細胞外小胞/エキソソームを介した乳腺上皮細胞と乳房管腔細胞との間の乳腺形成能の伝達…
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Developmental Biology

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JoVE Journal Developmental Biology
細胞外小胞/エキソソームを介した乳腺上皮細胞と乳房管腔細胞との間の乳腺形成能の伝達
Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens
Journal (Developmental Biology)
Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens
Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
Journal (Developmental Biology)
Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
 
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細胞外小胞/エキソソームを介した乳腺上皮細胞と乳房管腔細胞との間の乳腺形成能の伝達

Article DOI: 10.3791/55736-v • 09:59 min • June 3rd, 2017
June 3rd, 2017 •
*1, *1, 1, 1, 1
1Institute of Cellular and System Medicine, National Health Research Institutes
* These authors contributed equally

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このプロトコルは、非接着性/間葉性乳房上皮細胞からの細胞外小胞（EVs）/エキソソームの精製、定量化および特徴づけ方法、ならびにこれらを用いて乳腺上皮細胞に乳腺形成能を転移させる方法を記載する。幹様乳腺上皮細胞に由来するEV /エキソソームは、この細胞特性をEV /エキソソームを摂取する細胞に移入することができる。

Tags

発達生物学、第124号、細胞外小胞、エキソソーム、幹細胞、ナノ粒子追跡分析、超遠心分離、乳腺、密度勾配
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