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Journal / Developmental Biology / 通过细胞外囊泡/外来体转移乳腺基质上皮细胞和乳腺腔细胞之间的乳腺形成能力…
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JoVE Journal Developmental Biology
通过细胞外囊泡/外来体转移乳腺基质上皮细胞和乳腺腔细胞之间的乳腺形成能力
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通过细胞外囊泡/外来体转移乳腺基质上皮细胞和乳腺腔细胞之间的乳腺形成能力

Article DOI: 10.3791/55736-v • 09:59 min • June 3rd, 2017
June 3rd, 2017 •
*1, *1, 1, 1, 1
1Institute of Cellular and System Medicine, National Health Research Institutes
* These authors contributed equally

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该方案描述了从非贴壁/间充质乳腺上皮细胞中纯化，定量和表征细胞外囊泡（EVs）/外来体的方法，并使用它们将乳腺形成能力转移到乳腺上皮细胞。衍生自茎状乳腺上皮细胞的EVs /外来体可以将该细胞特性转移到摄入EV /外来体的细胞中。

Tags

发育生物学，第124期，细胞外囊泡，外来体，干细胞，纳米粒子跟踪分析，超速离心，乳腺，密度梯度
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