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Måling af glukoseoptagelse og reaktion på insulinstimulering i
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Måling af glukoseoptagelse og reaktion på insulinstimulering i

Article DOI: 10.3791/55743-v • 08:03 min • June 25th, 2017
June 25th, 2017 •
1, 1, 1, 2, 3, 1, 1
1CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva

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Ved denne metode dyrkes humane primære muskelceller in vitro for at opnå differentierede myotuber, og glukoseoptagelseshastigheder måles. Vi giver en detaljeret protokol til kvantificering af satser i basale og insulinstimulerede tilstande ved brug af radioaktivt mærket [3H] 2-deoxy-D-glucose.

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Cellular Biology Skeletmuskulatur glukosemetabolismen insulin signalering human myotube insulinfølsomhed radioaktivitet
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