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JoVE Journal
Biology

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葡萄糖摄取测量和对胰岛素刺激的反应
 
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葡萄糖摄取测量和对胰岛素刺激的反应

Article DOI: 10.3791/55743-v 08:03 min June 25th, 2017
June 25th, 2017

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Summary

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Click here for the English version.

在这种方法中,人原代肌细胞在体外培养以获得分化的肌管,并测量葡萄糖摄取率。我们提供了一个详细的方案,使用放射性标记的[ 3 H] 2-脱氧-D-葡萄糖来量化基础和胰岛素刺激状态的速率。

Tags

细胞生物学,第124期,骨骼肌,葡萄糖代谢,胰岛素信号,人肌管,胰岛素敏感性,放射性
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