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Journal / Biology / Påvisning af østrogene ligander i produkter til…
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JoVE Journal Biology
Påvisning af østrogene ligander i produkter til personlig pleje ved hjælp af en gær østrogen skærm optimeret for Undergraduate undervisning laboratorium
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Påvisning af østrogene ligander i produkter til personlig pleje ved hjælp af en gær østrogen skærm optimeret for Undergraduate undervisning laboratorium

Article DOI: 10.3791/55754-v • 14:13 min • January 1st, 2018
January 1st, 2018 •
1,2, 2,3, 4, 5, 4,6, 4
1Department of Biology, University of the South, 2School of Biological Sciences, Louisiana Tech University, 3School of Medicine, Louisiana State University Health Sciences Center, 4Department of Biology, Furman University, 5Department of Computer Science, Louisiana Tech University, 6Clemson University

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Denne artikel præsenterer en optimeret gær østrogen skærmen for kvantificering ligander i personlig plejeprodukter (PCPs) der binder østrogen receptorer alpha (ERα) og/eller beta (ERβ). Metoden indeholder to kolorimetriske substrat muligheder, en seks-dages nedkølet inkubation til brug i undergraduate kurser og statistiske værktøjer til dataanalyse.

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Cellebiologi sag 131 gær østrogen skærm bachelor hormonforstyrrelser østrogene personlige plejeprodukter miljømæssige østrogener ESR1 ESR2 østrogen receptor ortho-nitrophenylfosfat-β-D-galactopyranoside chlorphenol rød-β-D-galactopyranoside
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