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Transiënte expressie en cellulaire lokalisatie van recombinante eiwitten in gekweekte insectencellen
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Transiënte expressie en cellulaire lokalisatie van recombinante eiwitten in gekweekte insectencellen

Article DOI: 10.3791/55756-v • 12:09 min • April 20th, 2017
April 20th, 2017 •
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1U.S. Arid Land Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture

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Nonlytic insectencel expressiesystemen onderbenut productie, cellulair transport / lokalisatie en recombinante eiwitten functionele analyse. We beschrijven werkwijzen voor expressievectoren en transiënte daaropvolgende eiwitexpressie genereren handel verkrijgbaar Lepidoptera cellijnen. De co-localisatie van Bemisia tabaci aquaporins met fluorescente subcellulaire markereiwitten wordt gepresenteerd.

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Cellular Biology heterologe eiwitexpressie insecten celkweek, Sf9 transfectie cellulaire lokalisatie confocale microscopie fluorescerende eiwitten live cell imaging, Aquaporine
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