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Biochemistry

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JoVE Journal Biochemistry
Optimiertes Protokoll zur Extraktion von Proteinen aus dem menschlichen Mitralventil
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Optimiertes Protokoll zur Extraktion von Proteinen aus dem menschlichen Mitralventil

Article DOI: 10.3791/55762-v • 09:13 min • June 14th, 2017
June 14th, 2017 •
1, 2, 1, 1, 1, 1, 2,3,4
1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

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Die Proteinzusammensetzung des menschlichen Mitralklappens ist noch teilweise unbekannt, weil seine Analyse durch niedrige Zellularität und damit durch eine geringe Proteinbiosynthese kompliziert wird. Diese Arbeit liefert ein Protokoll zur effizienten Extraktion von Protein für die Analyse des Mitralklappenproteoms.

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Biochemie Ausgabe 124 menschliches normales Mitralklappe Proteinextraktion Gewebehomogenisierung Proteinanalyse Proteomik Biochemie
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