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JoVE Journal Bioengineering
Ett integrerat System att distans utlösa intracellulära signaltransduktion genom uppkonvertering nanopartikel-medierad Kinase ljusaktivering
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Ett integrerat System att distans utlösa intracellulära signaltransduktion genom uppkonvertering nanopartikel-medierad Kinase ljusaktivering

Article DOI: 10.3791/55769-v • 11:20 min • August 30th, 2017
August 30th, 2017 •
1,2, 1, 1,2, 3, 1,2, 1
1Institute of Chemistry, Academia Sinica, 2Department of Chemistry, National Taiwan University, 3Department of Materials and Mineral Resources Engineering, National Taipei University of Technology

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I detta protokoll, Bur proteinkinas A (PKA), en cellulära signaltransduktion bioeffector, var orörlig på en nanopartikel yta, microinjected i cytosolen och aktiveras av matas ut UV-ljus från nära infrarött (NIR) bestrålning, förmå nedströms stress fiber sönderfall i cytosolen.

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Bioteknik fråga 126 Caged enzym cellulära svar Mikroskop fotolys proteinkinas A uppkonvertering nanopartiklar ljusaktivering
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