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Journal / Genetics / Omfattende DNA-methyleringsanalyse ved anvendelse …
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Genetics

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JoVE Journal Genetics
Omfattende DNA-methyleringsanalyse ved anvendelse af en methyl-CpG-bindingsdomæne-capture-baseret metode i patienter med kronisk lymfocytisk leukæmi
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Omfattende DNA-methyleringsanalyse ved anvendelse af en methyl-CpG-bindingsdomæne-capture-baseret metode i patienter med kronisk lymfocytisk leukæmi

Article DOI: 10.3791/55773-v • 13:21 min • June 16th, 2017
June 16th, 2017 •
1, 2
1Department of Medical Genetics, Institute of Biomedicine, Gothenburg University, 2Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Gothenburg University

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Dette arbejde beskriver en optimeret methyl-CpG-bindingsdomæne (MBD) sekventeringsprotokol og en beregningsrørledning til identifikation af differentielt methylerede CpG-rige regioner hos patienter med kronisk lymfocytisk leukæmi (CLL).

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Genetik udgave 124 DNA-methylering methyl CpG DNA-bindingsdomæneprotein differentielt methylerede regioner næste generations sekventering proteinkoding og ikke-kodende gener kronisk lymfocytisk leukæmi
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