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Bioengineering

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JoVE Journal Bioengineering
Isolamento de células de gânglio retiniano murino primário (RGCs) por citometria de fluxo
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Isolamento de células de gânglio retiniano murino primário (RGCs) por citometria de fluxo

Article DOI: 10.3791/55785-v • 11:01 min • July 5th, 2017
July 5th, 2017 •
1, 1, 1, 1, 1, 2, 1,3,4, 1,2
1Department of Ophthalmology, Hamilton Eye Institute, University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

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Milhões de pessoas sofrem de doenças degenerativas da retina que resultam em cegueira irreversível. Um elemento comum de muitas dessas doenças é a perda de células ganglionares da retina (RGCs). Este protocolo detalhado descreve o isolamento de RGC murinos primários por seleção positiva e negativa com citometria de fluxo.

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Bioengenharia Edição 125 células ganglionares da retina RGC citometria de fluxo neurodegeneração glaucoma envelhecimento
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