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JoVE Journal Bioengineering
Isolement des cellules de ganglion rétiniennes murines primaires (RGC) par cytométrie de flux
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Isolement des cellules de ganglion rétiniennes murines primaires (RGC) par cytométrie de flux

Article DOI: 10.3791/55785-v • 11:01 min • July 5th, 2017
July 5th, 2017 •
1, 1, 1, 1, 1, 2, 1,3,4, 1,2
1Department of Ophthalmology, Hamilton Eye Institute, University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

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Des millions de personnes souffrent de maladies dégénératives de la rétine qui entraînent une cécité irréversible. Un élément commun de nombreuses de ces maladies est la perte de cellules ganglionnaires de la rétine (RGC). Ce protocole détaillé décrit l'isolement des RGC murins primaires par sélection positive et négative avec cytométrie de flux.

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Bioengineering Numéro 125 cellules ganglionnaires de la rétine RGC cytométrie de flux neurodégénérescence glaucome vieillissement
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