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Journal / Bioengineering / Isolierung von primären murinen…
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JoVE Journal Bioengineering
Isolierung von primären murinen Retinal-Ganglion-Zellen (RGCs) durch Durchflusszytometrie
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Isolierung von primären murinen Retinal-Ganglion-Zellen (RGCs) durch Durchflusszytometrie

Article DOI: 10.3791/55785-v • 11:01 min • July 5th, 2017
July 5th, 2017 •
1, 1, 1, 1, 1, 2, 1,3,4, 1,2
1Department of Ophthalmology, Hamilton Eye Institute, University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

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Millionen von Menschen leiden an retinalen degenerativen Erkrankungen, die zu irreversibler Blindheit führen. Ein gemeinsames Element vieler dieser Krankheiten ist der Verlust von Netzhautganglienzellen (RGCs). Dieses detaillierte Protokoll beschreibt die Isolierung von primären murinen RGCs durch positive und negative Selektion mit Durchflusszytometrie.

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Bioengineering Netzhautganglienzellen RGC Durchflusszytometrie Neurodegeneration Glaukom Alterung
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