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Sectioning Mammary Gland Whole Mounts for Lesion Identification

Article DOI: 10.3791/55796-v • 09:22 min • July 24th, 2017
July 24th, 2017 •
1,2, 3, 4, 2
1Curriculum in Toxicology, University of North Carolina at Chapel Hill, 2National Toxicology Program Laboratory (NTPL), DNTP, National Institute of Environmental Health Sciences, 3Cellular and Molecular Pathology Branch, DNTP, National Institute of Environmental Health Sciences, 4Charles River Laboratories Inc.

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Summary
Automatic Translation

We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.

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Sectioning Mammary Gland Whole Mount Lesion Identification Stained Sections Microscopic Level Histopathological Identification Technique Formalin Fits H And E Stain Whole Mount Phenotypes Visual Demonstration Removal And Embedding Steps Delicate Gland Thin Friable Nature Cover Slips Mounting Medium Glass Staining Jar Xylene Incubate Overnight Forceps
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