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Bioengineering

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JoVE Journal Bioengineering
Forudsigelse af gensvulmning gennem spatiotemporal kontrol af siRNA-frigivelse fra fotoresvarende polymere nanocarriers
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Forudsigelse af gensvulmning gennem spatiotemporal kontrol af siRNA-frigivelse fra fotoresvarende polymere nanocarriers

Article DOI: 10.3791/55803-v • 11:53 min • July 21st, 2017
July 21st, 2017 •
1, 1,2, 1
1Department of Chemical and Biomolecular Engineering, University of Delaware, 2Department of Materials Science and Engineering, University of Delaware

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Vi præsenterer en ny metode, der bruger fotobesvarende blokcopolymerer til mere effektiv spatiotemporal styring af gentympning uden påviselige off-target-effekter. Endvidere kan ændringer i genekspression forudsiges ved anvendelse af straightforward siRNA frigivelsesassays og simpel kinetisk modellering.

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Bioengineering udgave 125 Polyplex kinetisk modellering blokcopolymer nukleinsyrefrigivelse fluorescenskorrelationsspektroskopi cellepatrering RNAi lysfølsom
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