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Journal / Developmental Biology / Middelgrote voorbereiding van
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Developmental Biology

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JoVE Journal Developmental Biology
Middelgrote voorbereiding van
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Journal (Developmental Biology)
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines
Journal (Developmental Biology)
In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines
 
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Middelgrote voorbereiding van

Article DOI: 10.3791/55804-v • 09:16 min • May 30th, 2017
May 30th, 2017 •
1, 1, 1, 2, 1
1Biology Department, University of Massachusetts Boston, 2Department of Bioengineering, University of California, San Diego

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Het doel van dit protocol is om een ​​eenvoudige en goedkope aanpak te geven voor het verzamelen van Drosophila embryo's op middelgrote schaal (0,5-1 g) en het bereiden van eiwit extracten die kunnen worden toegepast in stroomafwaartse proteomische toepassingen, zoals affiniteitszuivering-massaspectrometrie (AP-MS ).

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Ontwikkelingsbiologie Uitgave 123 extract lysaat eiwit, Fruitvlieg embryo proteomics middelgrote schaal zuivering kooi
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