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JoVE Journal Genetics
Toveis retroviral integrasjonssted PCR-metodikk og kvantitativ dataanalyse arbeidsflyt
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Toveis retroviral integrasjonssted PCR-metodikk og kvantitativ dataanalyse arbeidsflyt

Article DOI: 10.3791/55812-v • 12:53 min • June 14th, 2017
June 14th, 2017 •
*1,2, *3, 1, 3, 4, 1,5, 6
1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)
* These authors contributed equally

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Dette manuskriptet beskriver eksperimentell prosedyre og programvareanalyse for en toveis integreringsstedanalyse som samtidig kan analysere oppstrøms og nedstrøms vektor-vert-kryss-DNA. Toveis PCR-produkter kan brukes til alle nedstrøms sekvenseringsplattformer. De resulterende dataene er nyttige for en høy gjennomstrømning, kvantitativ sammenligning av integrerte DNA-mål.

Tags

Genetikk Utgave 124 Retrovirus Integrasjonssteder Neste generasjons sekvensering Retroviral genterapi Stamceller Cancers Cellular barcoding Bioinformatics
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