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Journal / Genetics / 双向逆转录病毒整合位点PCR方法和定量数据分析工作流程…
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Genetics

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JoVE Journal Genetics
双向逆转录病毒整合位点PCR方法和定量数据分析工作流程
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双向逆转录病毒整合位点PCR方法和定量数据分析工作流程

Article DOI: 10.3791/55812-v • 12:53 min • June 14th, 2017
June 14th, 2017 •
*1,2, *3, 1, 3, 4, 1,5, 6
1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)
* These authors contributed equally

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该手稿描述了可以同时分析上游和下游载体 - 宿主连接DNA的双向整合位点测定的实验程序和软件分析。双向PCR产品可用于任何下游测序平台。所得数据对于综合DNA靶标的高通量，定量比较是有用的。

Tags

遗传学，第124期，逆转录病毒，整合位点，下一代测序，逆转录病毒基因治疗，干细胞，癌症，细胞条形码，生物信息学
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