Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

Tovejs Retroviral Integration Site PCR Metode og Quantitative Data Analysis Workflow
 
Click here for the English version

Tovejs Retroviral Integration Site PCR Metode og Quantitative Data Analysis Workflow

Article DOI: 10.3791/55812-v 12:53 min June 14th, 2017
June 14th, 2017

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

Dette manuskript beskriver den eksperimentelle procedure og softwareanalyse for et tovejs integrationssite-assay, der samtidigt kan analysere opstrøms og nedstrøms vektor-værtsforbindelses-DNA. Tovejsede PCR-produkter kan anvendes til enhver downstream-sekventeringsplatform. De resulterende data er nyttige til en høj-gennemløbs kvantitativ sammenligning af integrerede DNA-mål.

Tags

Genetik Udgave 124 Retrovirus Integrationssteder Næste generations sekventering Retroviral genterapi Stamceller Cancers Cellular barcoding Bioinformatics
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter